UC知道请帮我翻译一下````急急``
来源：百度知道 编辑：UC知道 时间：2024/06/07 16:17:27
Aims: The purpose of this study was to apply nucleic acid sequence-based amplification (NASBA) for the
detection of Salmonella enterica serovar Enteritidis (S. Enteritidis) in representative foods.
Methods and Results: A previously reported primer and probe set based on mRNA sequences of the dnaK gene
of Salmonella were used in this study. To test for possible food matrix inhibition and assay detection limits, 25-g
samples of representative food commodities (fresh meats, poultry, fish, ready-to-eat salads and bakery products)
were pre-enriched with and without S. Enteritidis inoculation. The NucliSens Basic Kit, supplemented with
enzymes from various other commercial sources, was used for RNA isolation, NASBA amplification and
electrochemiluminescent (ECL) detection. The end point detection limit of the NASBA–ECL assay was equivalent
to 101 CFU of S. Enteritidis per amplification reaction. When the assay was tested on noncontaminated foods,
detection of Salmonella enterica serovar Enteritidis (S. Enteritidis) in representative foods.
Methods and Results: A previously reported primer and probe set based on mRNA sequences of the dnaK gene
of Salmonella were used in this study. To test for possible food matrix inhibition and assay detection limits, 25-g
samples of representative food commodities (fresh meats, poultry, fish, ready-to-eat salads and bakery products)
were pre-enriched with and without S. Enteritidis inoculation. The NucliSens Basic Kit, supplemented with
enzymes from various other commercial sources, was used for RNA isolation, NASBA amplification and
electrochemiluminescent (ECL) detection. The end point detection limit of the NASBA–ECL assay was equivalent
to 101 CFU of S. Enteritidis per amplification reaction. When the assay was tested on noncontaminated foods,
目的：本研究的目的是为了适用于核酸序列为基础的扩增（ nasba ）为
检测沙门杆菌肠炎（沙门氏菌）的代表食品。
方法和结果：以前所报道的引物和探针一套基于基因序列的基因dnak
沙门氏菌被用于这项研究。测试食物可能抑制矩阵检测和检测限， 25庚
样本的代表性食品商品（新鲜肉类，家禽，鱼，即食沙拉和面包制品）
被预富集与无沙门氏菌疫苗注射。 nuclisens的基本工具，并辅以
酶其他各种商业渠道，是用于核糖核酸分离， nasba放大
电化学发光（发光）检测。终点检测限的nasba -化学发光检测当量
101祖的沙门氏菌每扩增反应。当检测检验扬中食品
没有食物矩阵产生假阳性结果。一些粮食矩阵抑制nasba -发光
除非反应相关核糖核酸稀释10倍，前扩增。
结论：所有食品检验，阳性发光信号后，取得了18 h的预富集
随后nasba在初次接种水平102和101的cfu每25克食物样本。
意义和影响的研究：这个快速，半自动化的检测方法有可能用于在
食品，农业和公共卫生部门。
这么长 请个英语老师给你翻译去...........~!